出芽短梗霉DBS66产单宁酶的优化

Optimization of tannase production by Aureobasidium pullulans DBS66.

作者信息

Banerjee Debdulal, Pati Bikas R

机构信息

Department of Microbiology, Vidyasagar University, Midnapore-721102, West Bengal, India.

出版信息

J Microbiol Biotechnol. 2007 Jun;17(6):1049-53.

PMID:18050927

Abstract

Tannase production by Aureobasidium pullulans DBS66 was optimized. The organism produced maximum tannase in the presence of 1% tannic acid after 36 h. Maximum gallic acid accumulation was observed within 36 h and tannic acid in the fermented broth was completely degraded after 42 h of growth. Glucose had a stimulatory effect on tannase synthesis at 0.1% (w/v) concentration. The organism showed maximum tannase production with (NH4)2HPO4 as nitrogen source. Shaking speed of 120 rpm and 50-ml broth volume have been found to be suitable for maximum tannase production.

摘要

对出芽短梗霉DBS66产单宁酶的条件进行了优化。该菌株在含有1%单宁酸的条件下培养36小时后可产生最大量的单宁酶。在36小时内观察到没食子酸积累量最大，生长42小时后发酵液中的单宁酸被完全降解。葡萄糖在浓度为0.1%（w/v）时对单宁酶的合成有促进作用。该菌株以磷酸氢二铵作为氮源时可产生最大量的单宁酶。已发现120转/分钟的振荡速度和50毫升的发酵液体积适合于最大量地生产单宁酶。