Heo Seok, Lee Si-Kyung, Lee Chi-Ho, Min Sang-Gi, Park Jong-Seok, Kim Hee-Yun
Department of Applied Biology and Chemistry, Konkuk University, Seoul 143-701, Korea.
J Microbiol Biotechnol. 2007 Apr;17(4):663-7.
Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange chromatography, and the specific activity was increased 1,791 fold. The bacteriocin (6,400 AU/ ml) was inoculated with L. monocytogenes V7 and incubated for 0.5 h, 1 h, 3 h, and 6 h. The bacteriocin was found to destroy most of the cell wall and released most of the inclusions in the cells after 6 h of incubation. These results suggest that the bactericidal effect of the bacteriocin was due to bacterial lysis.
嗜酸乳杆菌产生的细菌素可杀死单核细胞增生李斯特菌。通过透射电子显微镜研究了该细菌素对单核细胞增生李斯特菌V7的杀菌作用方式。使用微珠和阳离子交换色谱从无细胞提取物中部分纯化细菌素，其比活性提高了1791倍。将细菌素（6400 AU/ml）与单核细胞增生李斯特菌V7接种并孵育0.5小时、1小时、3小时和6小时。孵育6小时后发现细菌素破坏了大部分细胞壁并释放了细胞中的大部分内含物。这些结果表明细菌素的杀菌作用是由于细菌裂解。
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