Nian Hua, Qin Lu-Ping, Chen Wan-Sheng, Zhang Qiao-Yan, Zheng Han-Chen, Wang Yin
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Acta Pharmacol Sin. 2006 Jun;27(6):728-34. doi: 10.1111/j.1745-7254.2006.00328.x.
To investigate the protective effect of steroidal saponins from Anemarrhena asphodeloides (ATS) on ovariectomy (OVX)-induced bone loss.
Sprague-Dawley rats were divided into sham and OVX groups. The OVX rats were treated with vehicle, nylestriol or steroidal saponins extract for 12 weeks. Serum calcium, phosphorus, estradiol (E(2)), osteocalcin concentration and serum alkaline phosphatase activity were measured. Bone density was assayed by dual-energy X-ray absorptiometry. The undecalcified longitudinal proximal tibial metaphysical (PTM) sections were cut and stained for histomorphometric analysis of the bone.
In OVX rats, alkaline phosphatase activities in serum were markedly increased and concentrations of osteocalcin were decreased by ATS treatment, which had no influence on the body weight. Meanwhile, atrophy of the uterus and descent of bone mineral density (BMD) was suppressed by treatment with ATS. In addition, ATS completely corrected the decreased the concentration of calcium and E(2) in serum observed in OVX rats. Histological results showed ATS prevented decreases in trabecular thickness and increases in trabecular separation of proximal tibia metaphysis (PTM) in OVX rats. However, it did not alter osteoclast number in OVX rats. Moreover, ATS (300 mg/kg) had a remarkable effect on promoting bone formation action in OVX rats. Nylestriol treatment decreased the bone formation rate and mineral apposition rate.
An adequate supply of steroidal saponins of Anemarrhena asphodeloides prevented OVX-induced bone loss in rats through the promotion of bone formation but not the inhibition of bone resorption.
研究知母甾体皂苷（ATS）对去卵巢（OVX）诱导的大鼠骨质流失的保护作用。
将Sprague-Dawley大鼠分为假手术组和去卵巢组。对去卵巢大鼠用赋形剂、尼尔雌醇或甾体皂苷提取物处理12周。检测血清钙、磷、雌二醇（E₂）、骨钙素浓度和血清碱性磷酸酶活性。采用双能X线吸收法测定骨密度。切取未脱钙的胫骨近端干骺端（PTM）纵切片并染色，用于骨组织形态计量分析。
在去卵巢大鼠中，ATS处理可使血清碱性磷酸酶活性显著升高，骨钙素浓度降低，且对体重无影响。同时，ATS处理可抑制子宫萎缩和骨矿物质密度（BMD）下降。此外，ATS完全纠正了去卵巢大鼠血清中钙和E₂浓度的降低。组织学结果显示，ATS可防止去卵巢大鼠胫骨近端干骺端（PTM）小梁厚度减小和小梁间距增加。然而，它并未改变去卵巢大鼠破骨细胞数量。此外，ATS（300mg/kg）对促进去卵巢大鼠的骨形成作用显著。尼尔雌醇处理降低了骨形成率和矿物质沉积率。
充足的知母甾体皂苷供应可通过促进骨形成而非抑制骨吸收来预防去卵巢诱导的大鼠骨质流失。
