Nonaka Hiroshi, Tsukiji Shinya, Ojida Akio, Hamachi Itaru
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan.
J Am Chem Soc. 2007 Dec 26;129(51):15777-9. doi: 10.1021/ja074176d. Epub 2007 Dec 5.
We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.
我们在此描述了一种使用肽标签和合成分子探针的互补识别对进行蛋白质共价标记的新方法。通过标签位点与探针之间基于选择性分子识别的反应，实现了标签融合蛋白的快速且特异性的共价标记。该方法的优点包括标签融合蛋白的功能修饰简便以及标记特异性高，这在各种条件下甚至细胞内的标记实验中都得到了证明。
