通过结合诱导的酰基转移反应实现FLAG标签选择性共价蛋白质标记
FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction.
作者信息
Nonaka Hiroshi, Fujishima Sho-hei, Uchinomiya Sho-hei, Ojida Akio, Hamachi Itaru
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
出版信息
Bioorg Med Chem Lett. 2009 Dec 1;19(23):6696-9. doi: 10.1016/j.bmcl.2009.09.122. Epub 2009 Oct 3.
PMID:19837586
Abstract
A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.
摘要
本研究新开发了一种FLAG标签选择性蛋白质标记方法。合成的镍配合物探针与FLAG标签之间的选择性结合与酰基转移反应相结合，能够对FLAG肽和FLAG标签融合蛋白进行位点选择性共价修饰。
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