来自猿猴病毒转化的小鼠细胞的膜囊泡对钠刺激的α-氨基异丁酸的转运
Sodium-stimulated alpha-aminoisobutyric acid transport by membrane vesicles from simian virus-transformed mouse cells.
作者信息
Hamilton R T, Nilsen-Hamilton M
出版信息
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1907-11. doi: 10.1073/pnas.73.6.1907.
Abstract
Uptake of alpha-aminoisobutyric acid, by membrane vesicles derived principally from the plasma membrane and endoplasmic reticulum of mouse 3T3 cells transformed by simian virus 40, is stimulated by sodium chloride. Both in the presence and absence of Na+ uptake is time-dependent and osmotically sensitive. The Na+-stimulated uptake is inhibited by other amino acids. The kinetics of transport of alpha-aminoisobutyric acid are shown to be biphasic both in whole cells and in the membrane vesicles. Only the high affinity system is stimulated by sodium in the membrane vesicles. These results demonstrate that observations made on living cells correlate with observations made on isolated membrane vesicles, and indicate that these membrane vesicles have retained the cellular amino acid transport system functionally intact.
摘要
源自经猿猴病毒40转化的小鼠3T3细胞的主要质膜和内质网的膜泡对α-氨基异丁酸的摄取受氯化钠刺激。无论有无Na+，摄取均呈时间依赖性且对渗透压敏感。Na+刺激的摄取受其他氨基酸抑制。α-氨基异丁酸的转运动力学在全细胞和膜泡中均显示为双相。在膜泡中只有高亲和力系统受钠刺激。这些结果表明，对活细胞的观察结果与对分离膜泡的观察结果相关，并表明这些膜泡在功能上保留了完整的细胞氨基酸转运系统。
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