Madazli Riza, Kucur Mine, Gezer Altay, Isman Ferruh, Bulut Berk
Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul, Turkey.
Int J Gynaecol Obstet. 2008 Mar;100(3):239-43. doi: 10.1016/j.ijgo.2007.09.033. Epub 2007 Dec 3.
To compare macrophage activation in normal and pre-eclamptic pregnancies by determining YKL-40 concentration and chitotriosidase activity in maternal and cord serum.
In this prospective case-control study samples of maternal peripheral blood and umbilical venous blood were collected from 28 pre-eclamptic and 24 normotensive pregnant women and their newborns. YKL-40 concentration and chitotriosidase activity were determined by enzyme-linked immunoassay and fluorometry, respectively.
Chitotriosidase activity in maternal and cord serum and YKL-40 concentration in cord serum were significantly higher in pre-eclamptic pregnancies (P<0.001), but there was no significant difference in maternal serum levels of YKL-40 between the case and control groups (P>0.05). There was a significant positive correlation between diastolic blood pressure and (1) chitotriosidase activity in both maternal and cord serum and (2) cord serum concentration of YKL-40 (r=0.61, r=0.84, and r=0.58, respectively).
This study may be the first to demonstrate maternal and fetal macrophage activation in pre-eclampsia.
通过测定母血和脐血血清中YKL-40浓度及壳三糖苷酶活性，比较正常妊娠和子痫前期妊娠中巨噬细胞的激活情况。
在这项前瞻性病例对照研究中，收集了28例子痫前期孕妇、24例血压正常孕妇及其新生儿的母体外周血和脐静脉血样本。分别采用酶联免疫吸附测定法和荧光测定法测定YKL-40浓度及壳三糖苷酶活性。
子痫前期妊娠中母血和脐血血清中的壳三糖苷酶活性以及脐血血清中的YKL-40浓度显著更高（P<0.001），但病例组和对照组母血血清YKL-40水平无显著差异（P>0.05）。舒张压与（1）母血和脐血血清中的壳三糖苷酶活性以及（2）脐血血清YKL-40浓度之间存在显著正相关（r分别为0.61、0.84和0.58）。
本研究可能首次证实在子痫前期存在母胎巨噬细胞激活。
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