通过荧光原位杂交技术快速检测血培养中产超广谱β-内酰胺酶的肺炎克雷伯菌
Rapid detection of ESBL-producing Klebsiella pneumoniae in blood cultures by fluorescent in-situ hybridization.
作者信息
Palasubramaniam Selvi, Muniandy Sekaran, Navaratnam Parasakthi
机构信息
Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
J Microbiol Methods. 2008 Jan;72(1):107-9. doi: 10.1016/j.mimet.2007.10.008. Epub 2007 Dec 3.
Multi-resistant Enterobacteriaceae pose a serious threat of hospital acquired infections and their rapid identification is important for better clinical outcome. This study describes the rapid identification of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae of the sulphydryl variable-type by fluorescent in-situ hybridization. The method which rapidly identifies the target genes within 1 h could be a potentially rapid bacterial diagnostic tool.
多重耐药肠杆菌科细菌对医院获得性感染构成严重威胁，快速鉴定它们对于改善临床结果很重要。本研究描述了通过荧光原位杂交快速鉴定产超广谱β-内酰胺酶的巯基可变型肺炎克雷伯菌。该方法可在1小时内快速鉴定目标基因，可能成为一种潜在的快速细菌诊断工具。
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