Coon C N, Luther L W, Cough J R
Proc Soc Exp Biol Med. 1976 Jun;152(2):175-7. doi: 10.3181/00379727-152-39354.
The effect of cyclic 3',5'-AMP and supplemental dietary glycine upon de novo synthesis of serine metabolic enzymes in chick livers were examined. Chicks fed crystalline amino acid diets containing 2% glycine had approximately twofold the activity in liver for 3-phosphoglycerate dehydrogenase and phosphoserine phosphatase compared to liver tissue from chicks fed diets lacking in dietary glycine. Chicks subjected to daily intraperitoneal injections of cyclic 3',5'-AMP and fed diets containing no dietary glycine contained biosynthetic enzyme activity similar to glycine-fed chicks suggesting a correlation between glycine and cyclic AMP for serine enzyme induction. The elevated enzyme activity in liver of chicks fed dietary glycine or injected with cyclic AMP was inhibited when chicks were also injected with actinomycin D indicating de novo synthesis of 3-phosphoglycerate dehydrogenase and phosphoserine phosphatase. Dietary glycine or cyclic AMP, however, did not change serine dehydratase and glycerate dehydrogenase activities in chick liver.
研究了环3',5'-腺苷酸（cAMP）和补充膳食甘氨酸对雏鸡肝脏中丝氨酸代谢酶从头合成的影响。饲喂含2%甘氨酸的结晶氨基酸日粮的雏鸡，其肝脏中3-磷酸甘油酸脱氢酶和磷酸丝氨酸磷酸酶的活性大约是饲喂缺乏膳食甘氨酸日粮的雏鸡肝脏组织的两倍。每天腹腔注射cAMP且饲喂不含膳食甘氨酸日粮的雏鸡，其生物合成酶活性与饲喂甘氨酸的雏鸡相似，这表明甘氨酸和cAMP在丝氨酸酶诱导方面存在相关性。当雏鸡同时注射放线菌素D时，饲喂膳食甘氨酸或注射cAMP的雏鸡肝脏中升高的酶活性受到抑制，这表明3-磷酸甘油酸脱氢酶和磷酸丝氨酸磷酸酶是从头合成的。然而，膳食甘氨酸或cAMP并未改变雏鸡肝脏中丝氨酸脱水酶和甘油酸脱氢酶的活性。
