Sanborn T A, Kowle R L, Sallach H J
Endocrinology. 1975 Oct;97(4):1000-7. doi: 10.1210/endo-97-4-1000.
A significant decrease in the specific activity of 3 enzymes of serine and one-carbon metabolism (3-phosphoglycerate dehydrgenase, phosphoserine phosphatase, and serine hydroxy-methyltransferase) was found in the rat prostate gland with castration. A single injection of testosterone propionate to rats 3 days after castration resulted in a significant increase in the 3 enzyme activities within 24 h. This increase in specific activity was maximal 72 h after injection of testosterone in the case of 3-phosphoglycerate dehydrogenase and serine hydroxymethyltransferase. When cycloheximide was administered in conjunction with testosterone, the increase in 3-phosphoglycerate dehydrogenase and serine hydroxy-methyltransferase activity was significantly reduced compared to injection of testosterone alone. N6, O2'-dibutyryl adenosine-3',5'-cyclic monophosphate [(Bu)2 cAMP] and theophylline injected at 8 h intervals to rats 3 days after castration failed to mimic the action of testosterone on these 3 enzymes 24 and 72 h after beginning injections. Castration had no effect on the specific activity of these enzymes in the kidney; however, 3-phosphoglycerate dehydrogenase was significantly diminished in the liver 6 days after castration. A single injection of testosterone to rats 3 days after castration restored the activity to sham-operated levels. Serine hydroxy-methyltransferase and phosphoserine phosphatase activity in the liver were unaffected 6 days after castration. Thus testosterone exerts a regulatory role on serine and one-carbon metabolism in the prostate and liver which (Bu), cAMP is unable to minic.
阉割后的大鼠前列腺中，丝氨酸和一碳代谢的3种酶（3-磷酸甘油酸脱氢酶、磷酸丝氨酸磷酸酶和丝氨酸羟甲基转移酶）的比活性显著降低。阉割3天后给大鼠单次注射丙酸睾酮，24小时内这3种酶的活性显著增加。对于3-磷酸甘油酸脱氢酶和丝氨酸羟甲基转移酶，注射睾酮后72小时比活性增加最大。当与睾酮联合给予环己酰亚胺时，与单独注射睾酮相比，3-磷酸甘油酸脱氢酶和丝氨酸羟甲基转移酶活性的增加显著降低。阉割3天后的大鼠每隔8小时注射N6,O2'-二丁酰腺苷-3',5'-环磷酸[(Bu)2 cAMP]和茶碱，在开始注射后24小时和72小时未能模拟睾酮对这3种酶的作用。阉割对肾脏中这些酶的比活性没有影响；然而，阉割6天后肝脏中的3-磷酸甘油酸脱氢酶显著减少。阉割3天后给大鼠单次注射睾酮可使活性恢复到假手术水平。阉割6天后肝脏中的丝氨酸羟甲基转移酶和磷酸丝氨酸磷酸酶活性未受影响。因此，睾酮对前列腺和肝脏中的丝氨酸和一碳代谢发挥调节作用，而（Bu）2 cAMP无法模拟这种作用。
