来自柔弱马尾藻（褐藻门）的岩藻聚糖硫酸酯的特性及其抗凝血和诱导活性

Characterization of a fucoidan from Lessonia vadosa (Phaeophyta) and its anticoagulant and elicitor properties.

作者信息

Chandía N P, Matsuhiro B

机构信息

Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile.

出版信息

Int J Biol Macromol. 2008 Apr 1;42(3):235-40. doi: 10.1016/j.ijbiomac.2007.10.023. Epub 2007 Nov 4.

DOI:10.1016/j.ijbiomac.2007.10.023

PMID:18054382

Abstract

Blades of Lessonia vadosa (Phaeophyta) were extracted with 2% CaCl(2) solution, affording in 4.4% yield a polysaccharide which contained fucose and sulfate groups in the molar ratio 1.0:1.12. The high negative optical activity value (alpha(22)=-134.0 degrees ), FT-IR and NMR analysis suggest the presence of a fucoidan. (13)C NMR spectrum of the polysaccharide obtained by solvolytic desulfation of native fucoidan indicated the major presence of 1-->3 linked alpha-l-fucan. Depolymerization of the native fucoidan with H(2)O(2) in the presence of copper(II) acetate gave in 54.8% yield a fraction with 33.7% of sulfate content. The native fucoidan (MW 320,000) showed good anticoagulant activity whereas the radical depolymerized fraction (MW 32,000) presented a weak anticoagulant activity. These polysaccharides showed significant activation of phenylalanine-ammonia lyase (PAL), lipooxygenase (LOX) and glutathione-S-transferase (GST) defence enzyme activities in tobacco plants.

摘要

用2%氯化钙溶液提取柔弱马尾藻（褐藻门）的叶片，以4.4%的产率得到一种多糖，该多糖中岩藻糖和硫酸根的摩尔比为1.0:1.12。高的负旋光活性值（[α]D22 = -134.0°）、傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）分析表明存在岩藻聚糖。通过对天然岩藻聚糖进行溶剂解脱硫得到的多糖的碳-13核磁共振谱表明主要存在1→3连接的α-L-岩藻聚糖。在乙酸铜存在下，用过氧化氢对天然岩藻聚糖进行解聚，以54.8%的产率得到硫酸含量为33.7%的部分。天然岩藻聚糖（分子量320,000）表现出良好的抗凝血活性，而自由基解聚部分（分子量32,000）表现出较弱的抗凝血活性。这些多糖在烟草植株中显著激活了苯丙氨酸解氨酶（PAL）、脂氧合酶（LOX）和谷胱甘肽-S-转移酶（GST）防御酶的活性。