Bilan M I, Zakharova A N, Grachev A A, Shashkov A S, Nifant'ev N E, Usov A I
Bioorg Khim. 2007 Jan-Feb;33(1):44-53. doi: 10.1134/s1068162007010049.
A fucoidan containing L-fucose, sulfate, and O-acetyl groups at a molar ratio of 3 : 2 : 1, as well as minor amounts of xylose, galactose, and uronic acids was isolated from the brown alga Analipus japonicus collected in the Sea of Japan. The structures of the native polysaccharide and the products of its desulfation and deacetylation were studied by the methods of methylation, periodate oxidation, and NMR spectroscopy. It was shown that the polysaccharide molecule mainly consists of a linear carbohydrate chain of (1-->3)-linked alpha-L-fucopyranose residues, which bear numerous branches in the form of single alpha-L-fucopyranose residues (three branches at position 4 and one branch at position 2 per each ten residues of the main chain). Sulfate groups occupy positions 2 and (to a lesser extent) 4, most of the terminal nonreducing fucose residues being sulfated twice. The acetyl groups are located predominantly at positions 4. The structural role of minor monosaccharides was not established.
从采集于日本海的褐藻日本绳藻中分离出一种岩藻依聚糖，其L-岩藻糖、硫酸酯和O-乙酰基的摩尔比为3:2:1，还含有少量木糖、半乳糖和糖醛酸。通过甲基化、高碘酸盐氧化和核磁共振光谱法研究了天然多糖及其脱硫和脱乙酰产物的结构。结果表明，多糖分子主要由(1→3)连接的α-L-岩藻吡喃糖残基组成的线性碳水化合物链构成，该链带有许多以单个α-L-岩藻吡喃糖残基形式存在的分支（主链每十个残基在4位有三个分支，在2位有一个分支）。硫酸酯基团占据2位和（较少程度上的）4位，大多数末端非还原岩藻糖残基被硫酸化两次。乙酰基主要位于4位。尚未确定少量单糖的结构作用。
