Khil'kevich L V, Kurilo L F, Korogodina Iu V
Tsitol Genet. 1991 Nov-Dec;25(6):11-7.
On the 12th day of pregnancy CBA, 101/H and AKR mice were given thiophosphamide in a dose of 5 mg/kg bw, and were sacrificed on the 19th day of pregnancy. The action of thiophosphamide on embryonal ovaries and testes was studied by assaying for the population of oocytes and relative number of the different stages of meiotic prophase I; index of the degeneration germ cells; for the population of prospermatogonies and their degeneration; morphometric study of nucleus of nucleolus of prospermatogonies. A significant decrease of germ cells was found in male and female embryos on the 19th day of pregnancy after thiophosphamide injection. Interspecific differences were found as regards the number of germ cells and their proportion in health as well as in response to a single antenatal injection of thiophosphamide.
在妊娠第12天，给CBA、101/H和AKR小鼠按5毫克/千克体重的剂量注射硫磷酰胺，并在妊娠第19天处死。通过测定卵母细胞数量、减数分裂前期I不同阶段的相对数量、退化生殖细胞指数、精原细胞数量及其退化情况、精原细胞核仁的形态计量学研究，来研究硫磷酰胺对胚胎卵巢和睾丸的作用。注射硫磷酰胺后，在妊娠第19天的雄性和雌性胚胎中发现生殖细胞显著减少。在生殖细胞数量及其在健康状态下的比例以及对单次产前注射硫磷酰胺的反应方面，发现了种间差异。
