Tomoda T, Nomura I, Kurashige T, Kubonishi I, Miyoshi I, Sukenaga Y, Taniguchi T
Department of Pediatrics, Kochi Medical School, Japan.
Acta Haematol. 1991;86(4):183-8. doi: 10.1159/000204831.
We investigated the alteration of Cu,Zn-superoxide dismutase during erythroid and myeloid differentiation in order to elucidate its physiological significance in different types of cells. We measured enzyme activity and mRNA levels of superoxide dismutase in the process of differentiation to erythroid cells or myeloid cells. When human leukemia K562 cells are incubated in the presence of 80 microM hemin, benzidine-positive cells appear on day 1 and 80% of the cells become positive on day 5. During hemin-induced erythroid differentiation, Cu,Zn-superoxide dismutase activity increases 3.5-fold of the initial value and mRNA for Cu,Zn-superoxide dismutase increases prior to the activity to the same extent. On the other hand, when human promyelocytic leukemia HL-60 cells are incubated in the presence of 1.3% dimethyl sulfoxide, nitroblue tetrazolium-positive cells reach approximately 90% on day 5. During dimethyl sulfoxide-induced myeloid differentiation, the activity of Cu,Zn-superoxide dismutase decreases below 15% of the initial value on day 5 and mRNA for Cu,Zn-superoxide dismutase decreases as well. The results indicate that the synthesis of superoxide dismutase is linked with either the erythroid or myeloid differentiation program.
为阐明铜锌超氧化物歧化酶（Cu,Zn-superoxide dismutase）在不同类型细胞中的生理意义，我们研究了其在红系和髓系分化过程中的变化。我们检测了在分化为红系细胞或髓系细胞过程中超氧化物歧化酶的酶活性和mRNA水平。当人类白血病K562细胞在80微摩尔血红素存在的情况下培养时，第1天出现联苯胺阳性细胞，第5天80%的细胞变为阳性。在血红素诱导的红系分化过程中，铜锌超氧化物歧化酶活性增加至初始值的3.5倍，且铜锌超氧化物歧化酶的mRNA在活性增加之前也增加到相同程度。另一方面，当人类早幼粒细胞白血病HL-60细胞在1.3%二甲基亚砜存在的情况下培养时，第5天硝基蓝四氮唑阳性细胞达到约90%。在二甲基亚砜诱导的髓系分化过程中，第5天铜锌超氧化物歧化酶的活性降至初始值的15%以下，且铜锌超氧化物歧化酶的mRNA也下降。结果表明，超氧化物歧化酶的合成与红系或髓系分化程序相关。
