Peng Chang G, Damha Masad J
McGill University, Montreal, Quebec, Canada.
Nucleosides Nucleotides Nucleic Acids. 2007;26(8-9):1189-93. doi: 10.1080/15257770701527786.
We examined the ability of 2'-deoxy-2'-fluroarabinonucleoside 5'-triphosphates (2'F-araNTPs) to serve as substrates of various DNA polymerases. In addition, we also examined the ability of these polymerases to accept DNA-FANA (2'-deoxy-2'-fluoroarabinonucleic acids) chimeras as template strands while synthesizing a DNA or FANA-DNA complementary strand. We provide preliminary data demonstrating that 2'F-araNTPs are indeed substrates of several DNA polymerases, and that FANA-DNA chimeric templates are generally well recognized by these polymerase enzymes.
我们研究了2'-脱氧-2'-氟阿拉伯核苷5'-三磷酸（2'F-araNTPs）作为各种DNA聚合酶底物的能力。此外，我们还研究了这些聚合酶在合成DNA或FANA-DNA互补链时接受DNA-FANA（2'-脱氧-2'-氟阿拉伯核酸）嵌合体作为模板链的能力。我们提供的初步数据表明，2'F-araNTPs确实是几种DNA聚合酶的底物，并且FANA-DNA嵌合模板通常能被这些聚合酶很好地识别。
