Sharkin Y A, Alexandrova L A, Victorova L S, Kuznetsova E V, Krayevsky A A
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Biochem Mol Biol Int. 1995 Apr;35(5):1041-8.
Analogues of araNTPs carrying an azido or aminogroup instead of the 2' hydroxyl exhibited substrate properties towards several mammalian and viral DNA polymerases. At the same time, introduction of a bulky hydrophobic DNP group into the 2' position inactivated the compounds as substrates. HSV-1 and CMV DNA polymerases were an interesting exception: they effectively incorporated the modified nucleotide residues with DNP group into the 3'-termini of the DNA chain. This is a reliable distinction of these enzymes from cellular DNA polymerases.
携带叠氮基或氨基而非2'-羟基的araNTP类似物对几种哺乳动物和病毒DNA聚合酶表现出底物特性。与此同时，在2'位引入一个庞大的疏水性DNP基团会使这些化合物作为底物失活。单纯疱疹病毒1型（HSV-1）和巨细胞病毒（CMV）DNA聚合酶是一个有趣的例外：它们能有效地将带有DNP基团的修饰核苷酸残基掺入DNA链的3'-末端。这是这些酶与细胞DNA聚合酶的可靠区别。
