具有苯并呋喃结构的衍生化试剂开发的最新进展。

Recent progress in the development of derivatization reagents having a benzofurazan structure.

作者信息

Santa Tomofumi, Fukushima Takeshi, Ichibangase Tomoko, Imai Kazuhiro

机构信息

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

出版信息

Biomed Chromatogr. 2008 Apr;22(4):343-53. doi: 10.1002/bmc.945.

DOI:10.1002/bmc.945

PMID:18059058

Abstract

Chemical derivatization is often used to enhance the detectability of the target compounds and to improve the separation efficiency in high-performance liquid chromatography (HPLC). In this review, we describe the recent progress in the development of derivatization reagents having a benzofurazan structure, namely, the fluorogenic reagents, water-soluble reagents, reagents for the analysis of peptides and proteins, and reagents for mass spectrometric detection. The application of these reagents to bio-samples is also briefly described.

摘要

化学衍生化常用于提高目标化合物的可检测性，并改善高效液相色谱（HPLC）中的分离效率。在本综述中，我们描述了具有苯并呋喃嗪结构的衍生化试剂，即荧光试剂、水溶性试剂、用于肽和蛋白质分析的试剂以及用于质谱检测的试剂的最新进展。还简要介绍了这些试剂在生物样品中的应用。