Toyo'oka T
Department of Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Biomed Chromatogr. 1996 Nov-Dec;10(6):265-77. doi: 10.1002/(SICI)1099-0801(199611)10:6<265::AID-BMC602>3.0.CO;2-A.
Techniques for the resolution by liquid chromatography of racemic compounds based upon diastereomer formation with a fluorescent chiral reagent are outlined in this review. The tagging reagents for various functional groups, i.e. amine, carboxyl, hydroxyl and thiol, are evaluated in terms of optical purity, handling, flexibility, stability, sensitivity and selectivity. The applicabilities of the reagents to drugs and biologically important substances are included in the text. This review is limited to reagents with fluorophores and reagents that exhibit fluorescence.
本综述概述了基于与荧光手性试剂形成非对映异构体，通过液相色谱拆分外消旋化合物的技术。对用于各种官能团（即胺基、羧基、羟基和巯基）的标记试剂，从光学纯度、操作便利性、灵活性、稳定性、灵敏度和选择性等方面进行了评估。文中还介绍了这些试剂在药物和生物重要物质方面的应用。本综述仅限于含荧光团的试剂以及具有荧光的试剂。
