采用(R)-DBD-PyNCS衍生化后利用液相色谱-质谱联用技术测定人血清中的l-色氨酸和l-犬尿氨酸
Determination of l-tryptophan and l-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS.
作者信息
Ohashi Hayato, Iizuka Hideaki, Yoshihara Shunsuke, Otani Hayato, Kume Misato, Sadamoto Kiyomi, Ichiba Hideaki, Fukushima Takeshi
机构信息
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, Funabashi-shi, Chiba, Japan.
出版信息
Int J Tryptophan Res. 2013 Jul 21;6(Suppl 1):9-14. doi: 10.4137/IJTR.S11459. Print 2013.
Abstract
Concentrations of l-tryptophan (l-Trp) and its metabolite, l-kynurenine (l-KYN), in sera of 19 normal subjects (age: 23.6 ± 3.5 y, male: 8, female: 11) were determined by high-performance liquid chromatography with mass-spectrometric detection, following their derivatization with (R)-(-)-4-(N, N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). A significant positive correlation between l-Trp and l-KYN concentrations was observed (r = 0.532, P < 0.05). Serum l-Trp concentration in male subjects (95.65 ± 4.27 μM) was significantly higher than that in female subjects (79.20 ± 3.34 μM; P < 0.05), while no significant differences in l-KYN concentration or the l-KYN:l-Trp ratio were observed between male and female subjects.
摘要
采用高效液相色谱-质谱检测法，在19名正常受试者（年龄：23.6±3.5岁，男性8名，女性11名）的血清用（R）-（-）-4-（N，N-二甲基氨基磺酰基）-7-（3-异硫氰酸根合吡咯烷-1-基）-2,1,3-苯并二唑（DBD-PyNCS）衍生化后，测定血清中l-色氨酸（l-Trp）及其代谢产物l-犬尿氨酸（l-KYN）的浓度。观察到l-Trp与l-KYN浓度之间存在显著正相关（r = 0.532，P < 0.05）。男性受试者血清l-Trp浓度（95.65±4.27μM）显著高于女性受试者（79.20±3.34μM；P < 0.05），而男性和女性受试者之间l-KYN浓度或l-KYN:l-Trp比值无显著差异。
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