Masuda Kazumi, Hori Tatsuya, Tanabe Kai, Kano Yutaka, Hirayama Aki, Nagase Sohji
Department of Health Education, Faculty of Education, Kanazawa University, Kakuma-machi, Kanazawa City, Ishikawa, 920-1192, Japan.
Appl Physiol Nutr Metab. 2007 Dec;32(6):1097-104. doi: 10.1139/H07-073.
The present study was carried out to clarify the effect of oral administration of proanthocyanidin (PA) on radical-scavenging activity in muscle and plasma using electron spin resonance (ESR). Eight-week-old male Wistar rats were orally administered with 3 doses per day of 1 mL of 0.05% (PA0.05), 0.5% (PA0.5) or 5% (PA5) PA for 1 week. Control animals received the same volume of distilled water. We also examined the effect of a single dose of 0.5% PA. Blood and muscle were collected from rats 1 h after the final administration. Scavenging activity against superoxide anions in the plasma and m. soleus (Sol), m. plantaris (Pla), deep and surface areas of the m. gastrocnemius (GasD and GasS, respectively) and myocardium (Hrt) was determined using ESR with the spin trap, 5,5-dimethyl-1-pyrroline-N-oxide The scavenging activity in plasma for all groups given PA was 34%-44% higher than the control (p<0.05). The scavenging activity in Hrt, Sol and GasD increased by up to 50% compared with the control and tended to increase depending on the dose of PA (p<0.05). The impact of a single dose of PA was undetectable in all tissues. These results suggested that 1 week of oral PA improves the radical-scavenging activity in both plasma and muscle, especially in highly oxidative muscle. A single dose of PA was insufficient to improve the antioxidative capacity of muscle tissues.
本研究旨在利用电子自旋共振（ESR）阐明口服原花青素（PA）对肌肉和血浆中自由基清除活性的影响。8周龄雄性Wistar大鼠每天口服3次，每次1 mL的0.05%（PA0.05）、0.5%（PA0.5）或5%（PA5）PA，持续1周。对照动物接受相同体积的蒸馏水。我们还研究了单剂量0.5% PA的效果。在末次给药1小时后从大鼠采集血液和肌肉。使用自旋捕获剂5,5-二甲基-1-吡咯啉-N-氧化物通过ESR测定血浆以及比目鱼肌（Sol）、跖肌（Pla）、腓肠肌深层和表层区域（分别为GasD和GasS）及心肌（Hrt）中超氧阴离子的清除活性。给予PA的所有组血浆中的清除活性均比对照组高34%-44%（p<0.05）。与对照组相比，Hrt、Sol和GasD中的清除活性提高了50%，且有随PA剂量增加而升高的趋势（p<0.05）。单剂量PA对所有组织的影响均未检测到。这些结果表明，口服PA 1周可提高血浆和肌肉中的自由基清除活性，尤其是在高氧化肌肉中。单剂量PA不足以提高肌肉组织的抗氧化能力。
