Lai How-Yee, Lim Yau-Yan, Kim Kah-Hwi
School of Biosciences, Taylor's University, Jalan Taylor's, Subang Jaya, Selangor, Malaysia.
School of Science, Monash University Sunway Campus, Bandar Sunway, Petaling Jaya, Selangor, Malaysia.
Pharmacogn Mag. 2017 Jan-Mar;13(49):31-37. doi: 10.4103/0973-1296.197659.
Linn. (Blechnaceae), a fern, is traditionally used in the treatment of various ailments, such as skin diseases, stomach pain, urinary bladder complaints, and also as a female contraceptive. Previously, we reported a strong radical scavenging activity, antibacterial activity and cytotoxicity against HT29 colon cancer cells by aqueous extract of .
In this study, we attempted to isolate and identify the active compound from the aqueous extract of .
Aqueous extract of was subjected to repeated MCI gel chromatography, Sephadex-LH-20, Chromatorex C18 and semi-preparative high performance liquid chromatography and was characterized using nuclear magnetic resonance and electrospray ionization mass-spectrometry spectroscopic methods. Antioxidant activity was determined using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging assay. Antibacterial assays were conducted using disc diffusion whereas the minimum inhibitory concentration (MIC) and minimum bactericidal concentration were determined using the broth microdilution assay. Cytotoxicity was assessed using thiazolylblue tetrazoliumbromide.
A polymeric proanthocyanidin consisting of 2-12 epicatechin extension units and epigallocathecin terminal units linked at C4-C8 was elucidated. Bioactivity studies showed strong radical scavenging activity (IC = 5.6 ± 0.1 µg/mL), antibacterial activity (MIC = 31.3-62.5 µg/mL) against five gram-positive bacteria and selective cytotoxicity against HT29 colon cancer cells (IC = 7.0 ± 0.3 µg/mL).
According to our results, the proanthocyanidin of demonstrated its potential as a natural source of antioxidant with antibacterial and anti-cancer properties.
A bioactive proanthocyanidin was isolated from the aqueous extract of medicinal fern Linn and the structure was elucidated using NMR and ESI-MS spectral studies.The proanthocyanidin compound possessed strong radical scavenging activity (IC 5.6 ± 0.1 µg/mL)The proanthocyaniding compound showed bactericidal activity against five gram-positive bacteria inclusive of MRSA (minimum inhibitory concentration, MIC and minimum bactericidal concentration, MBC 31.3-62.5 µg/mL).The proanthocyanidin compound is strongly cytotoxic towards cancer cells HT29 (IC 7.0 ± 0.3 µg/mL), HepG2 (IC 16 µg/mL) and HCT116 (IC 20 µg/mL) while weakly cytotoxic towards the non-malignant Chang cells (IC 48 µg/mL). CC: Column chromatography, DP: degree of polymerization, DPPH: 2,2-diphenyl-1-picrylhydrazyl, ESI-MS: electronsprayionisation mass-spectrometry, MBC: Minimum bactericidal concentration, MIC: Minimum inhibitory concentration, MTT: Thiazolyl Blue Tetrazolium Bromide, MRSA: methicillin-resistant , NMR: nuclear magnetic resonance, TLC: thin layer chromatography, PD: prodelphinidin.
（某种蕨类植物）是一种蕨类植物，传统上用于治疗各种疾病，如皮肤病、胃痛、膀胱疾病，还用作女性避孕药。此前，我们报道了该植物水提取物具有较强的自由基清除活性、抗菌活性以及对HT29结肠癌细胞的细胞毒性。
在本研究中，我们试图从该植物水提取物中分离并鉴定活性化合物。
该植物水提取物经反复MCI凝胶柱色谱、葡聚糖凝胶LH - 20、Chromatorex C18和半制备高效液相色谱分离，并采用核磁共振和电喷雾电离质谱光谱法进行表征。抗氧化活性通过2,2 - 二苯基 - 1 - 苦基肼自由基清除试验测定。抗菌试验采用纸片扩散法，而最低抑菌浓度（MIC）和最低杀菌浓度则通过肉汤微量稀释法测定。细胞毒性通过噻唑蓝四氮唑溴盐法评估。
阐明了一种由2 - 12个表儿茶素延伸单元和表没食子儿茶素末端单元通过C4 - C8相连组成的聚合原花青素。生物活性研究表明其具有较强的自由基清除活性（IC = 5.6 ± 0.1 µg/mL）、对五种革兰氏阳性菌的抗菌活性（MIC = 31.3 - 62.5 µg/mL）以及对HT29结肠癌细胞的选择性细胞毒性（IC = 7.0 ± 0.3 µg/mL）。
根据我们的结果，该植物的原花青素显示出作为具有抗菌和抗癌特性的天然抗氧化剂来源的潜力。
从药用蕨类植物水提取物中分离出一种生物活性原花青素，并通过核磁共振和电喷雾电离质谱光谱研究阐明了其结构。该原花青素化合物具有较强的自由基清除活性（IC 5.6 ± 0.1 µg/mL）。该原花青素化合物对包括耐甲氧西林金黄色葡萄球菌在内的五种革兰氏阳性菌具有杀菌活性（最低抑菌浓度，MIC和最低杀菌浓度，MBC 31.3 - 62.5 µg/mL）。该原花青素化合物对癌细胞HT29（IC 7.0 ± 0.3 µg/mL）、HepG2（IC 16 µg/mL）和HCT116（IC 20 µg/mL）具有较强的细胞毒性，而对非恶性的Chang细胞（IC 48 µg/mL）细胞毒性较弱。 抄送：柱色谱，DP：聚合度，DPPH：2,2 - 二苯基 - 1 - 苦基肼，ESI - MS：电喷雾电离质谱，MBC：最低杀菌浓度，MIC：最低抑菌浓度，MTT：噻唑蓝四氮唑溴盐，MRSA：耐甲氧西林金黄色葡萄球菌，NMR：核磁共振，TLC：薄层色谱，PD：原飞燕草素
