Carvalho-Peixoto Jacqueline, Alves Robson Cardilo, Cameron Luiz-Claudio
Laboratório de Bioquímica de Proteínas, Universidade Federal do Estado do Rio de Janeiro, Brazil.
Appl Physiol Nutr Metab. 2007 Dec;32(6):1186-90. doi: 10.1139/H07-091.
Blood ammonia concentration increases during endurance exercise and has been proposed as a cause for both peripheral and central fatigue. We examined the impact of glutamine and (or) carbohydrate supplementation on ammonemia in high-level runners. Fifteen men in pre-competitive training ran 120 min (approximately 34 km) outdoors on 4 occasions. On the first day, the 15 athletes ran without the use of supplements and blood samples were taken every 30 min. After that, each day for 4 d before the next 3 exercise trials, we supplemented the athletes' normal diets in bolus with carbohydrate (1 g.kg(-1).d(-1)), glutamine (70 mg.kg(-1).d(-1)), or a combination of both in a double-blind study. Blood ammonia level was determined before the run and every 30 min during the run. During the control trial ammonia increased progressively to approximately 70% above rest concentration. Following supplementation, independent of treatment, ammonia was not different (p>0.05) for the first 60 min, but for the second hour it was lower than in the control (p<0.05). Supplementation in high-level, endurance athletes reduced the accumulation of blood ammonia during prolonged, strenuous exercise in a field situation.
在耐力运动期间血氨浓度会升高，并且有人提出这是外周和中枢疲劳的一个原因。我们研究了补充谷氨酰胺和（或）碳水化合物对高水平跑步者血氨水平的影响。15名处于赛前训练阶段的男性在户外进行了4次120分钟（约34公里）的跑步。第一天，15名运动员在不使用补充剂的情况下跑步，每30分钟采集一次血样。此后，在接下来的3次运动试验前的4天里，每天我们在一项双盲研究中给运动员的正常饮食中一次性补充碳水化合物（1克·千克⁻¹·天⁻¹）、谷氨酰胺（70毫克·千克⁻¹·天⁻¹）或两者的组合。在跑步前以及跑步过程中每30分钟测定血氨水平。在对照试验期间，氨水平逐渐升高至比静息浓度高约70%。补充后，无论采用何种处理方式，在前60分钟血氨水平无差异（p>0.05），但在第二个小时低于对照水平（p<0.05）。对高水平耐力运动员进行补充可减少在野外长时间剧烈运动期间血氨的积累。
