Koering M J, Danforth D R, Hodgen G D
Department of Anatomy, George Washington University, Washington, District of Columbia 20037.
Biol Reprod. 1991 Dec;45(6):890-7. doi: 10.1095/biolreprod45.6.890.
The purpose of this study was to examine the effect of an exogenous estrogen, diethylstilbestrol (DES), on follicle development in the ovary of a juvenile primate. The immature cynomolgus monkey (12-22 mo) was used as a model since ovaries at this age lack endogenous gonadotropin support but are capable of responding to exogenous hormonal stimulation. In addition, the pituitary gland receives virtually no GnRH stimulation and under these conditions lacks responsiveness to estrogen feedback. Two groups of three monkeys each received DES for 14 days. Members of the second group also were given GnRH antagonist to assure no GnRH action upon the gonadotropes. The left ovary of each monkey was removed just prior to Day 1 of DES treatment and served as the control. The right ovary was removed on Day 14 of treatment. Both ovaries from each monkey were prepared for evaluation by light microscopy. Results indicated that both the number of preantral follicles and the mean number of medium-sized (0.5-1 mm in diameter) developing antral follicles decreased significantly (p less than 0.05) in the DES-treated ovaries with no increase in early-atretic antral follicles. These data suggest that DES, at the amount administered, inhibits the growth of both preantral and medium-sized antral follicles in the primate. Whether these effects are manifest directly at the follicle level or are mediated by other mechanisms remains to be determined.
本研究的目的是检测外源性雌激素己烯雌酚（DES）对幼年灵长类动物卵巢卵泡发育的影响。选用未成熟食蟹猴（12 - 22月龄）作为模型，因为这个年龄段的卵巢缺乏内源性促性腺激素支持，但能够对外源性激素刺激作出反应。此外，垂体几乎未受到促性腺激素释放激素（GnRH）刺激，在此条件下对雌激素反馈缺乏反应性。两组，每组三只猴子，均接受14天的DES治疗。第二组的猴子还给予GnRH拮抗剂，以确保GnRH对促性腺细胞无作用。每只猴子在DES治疗第1天前切除左侧卵巢作为对照。在治疗第14天切除右侧卵巢。对每只猴子的双侧卵巢进行处理，以便通过光学显微镜进行评估。结果表明，在接受DES治疗的卵巢中，窦前卵泡数量和中等大小（直径0.5 - 1毫米）正在发育的窦卵泡平均数量均显著减少（p < 0.05），早期闭锁窦卵泡数量未增加。这些数据表明，所给予剂量的DES可抑制灵长类动物窦前卵泡和中等大小窦卵泡的生长。这些影响是直接在卵泡水平表现出来还是由其他机制介导，仍有待确定。
