Koering M J, Danforth D R, Hodgen G D
Department of Anatomy, George Washington University, District of Columbia 20037.
Biol Reprod. 1994 Mar;50(3):686-94. doi: 10.1095/biolreprod50.3.686.
The mechanism(s) that drives preantral and early antral follicle growth in the primate ovary is poorly understood. We previously reported that estrogen does not stimulate preantral or early antral follicle growth in juvenile primates. The purpose of the present study was to determine whether estrogen priming may play a role in enhancing FSH-stimulated follicle growth. Eight juvenile monkeys received implants on Day 1 of silastic capsules containing estradiol (or vehicle) to elevate circulating estradiol levels. Daily FSH injections were initiated on Day 4 and continued for up to 12 days. The left ovary was removed on Day 8 and served as a control. Ovulation was induced, and a luteal phase followed. On the first day of menses, the estradiol (vehicle) capsule-FSH protocol was repeated. The remaining ovary was removed on Day 8 of the second cycle. The number and size of all follicles in both ovaries were evaluated by light microscopy. Results indicate that estrogen priming in the first cycle did not enhance growth of preantral or antral follicles, but did result in fewer developing follicles > 1.0 mm in diameter, this was accompanied by an increase in early atretic antral follicles of similar size. In the second cycle, an even further reduction in number and size of developing antral follicles > 1.0 mm in diameter was observed. These data suggest that pretreatment with exogenous estrogen has an anti-follicular action on follicle growth in these primates.
灵长类动物卵巢中驱动窦前卵泡和早期窦卵泡生长的机制尚不清楚。我们之前报道过，雌激素不会刺激幼年灵长类动物的窦前卵泡或早期窦卵泡生长。本研究的目的是确定雌激素预处理是否可能在增强促卵泡激素（FSH）刺激的卵泡生长中发挥作用。八只幼年猴子在第1天接受含有雌二醇（或赋形剂）的硅橡胶胶囊植入，以提高循环雌二醇水平。在第4天开始每日注射FSH，并持续长达12天。在第8天切除左侧卵巢作为对照。诱导排卵并进入黄体期。在月经第一天，重复雌二醇（赋形剂）胶囊-FSH方案。在第二个周期的第8天切除剩余的卵巢。通过光学显微镜评估双侧卵巢中所有卵泡的数量和大小。结果表明，第一个周期中的雌激素预处理并未增强窦前卵泡或窦卵泡的生长，但确实导致直径>1.0mm的发育中卵泡数量减少，同时伴随类似大小的早期闭锁窦卵泡数量增加。在第二个周期中，观察到直径>1.0mm的发育中窦卵泡数量和大小进一步减少。这些数据表明，外源性雌激素预处理对这些灵长类动物的卵泡生长具有抗卵泡作用。
