Agrawal S B, Mishra Shweta
Department of Botany, Banaras Hindu University, Varanasi 221005, India.
Ecotoxicol Environ Saf. 2009 Feb;72(2):610-8. doi: 10.1016/j.ecoenv.2007.10.007. Epub 2007 Dec 3.
The impact of supplemental UV-B irradiation (sUV-B) and cadmium (Cd, 68 micromol kg(-1) soil) singly and in combination was studied on growth, pigments, enzymatic and non-enzymatic antioxidants, lipid peroxidation (LPO), uptake and translocation of Cd and yield of pea plants under field condition. Compared to the control, both the stresses individually and in combination led to in reductions in growth, photosynthetic pigments, ascorbic acid, catalase (CAT) activity and yield, whereas a reverse trend was observed for flavonoids, thiols and proline contents, superoxide dismutase (SOD) and peroxidase (POD) activities, and LPO. The uptake and translocation of Cd increased in different plant parts with duration of its treatment and also when applied with sUV-B. Combined treatment of sUV-B and Cd inhibited the growth and various metabolic processes antagonistically except the CAT activity which showed additive response. Further, CAT activity may be used as a bioindicator parameter to evaluate the individual and interactive effects of both the stresses.
在田间条件下，研究了补充紫外线B辐射（sUV-B）和镉（Cd，68微摩尔/千克土壤）单独及联合作用对豌豆植株生长、色素、酶促和非酶促抗氧化剂、脂质过氧化（LPO）、镉的吸收和转运以及产量的影响。与对照相比，两种胁迫单独及联合作用均导致生长、光合色素、抗坏血酸、过氧化氢酶（CAT）活性和产量降低，而类黄酮、硫醇和脯氨酸含量、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性以及LPO则呈现相反趋势。随着处理时间的延长，以及与sUV-B一起施用时，Cd在不同植物部位的吸收和转运增加。sUV-B和Cd的联合处理对生长和各种代谢过程具有拮抗抑制作用，但CAT活性表现为加性响应。此外，CAT活性可作为生物指示参数，用于评估两种胁迫的单独及交互作用。
