组胺受体（H(1)、H(2)和H(3)）在兔内淋巴囊中的表达：一项免疫组织化学研究。

Expression of histamine receptors (H(1), H(2), and H(3)) in the rabbit endolymphatic sac: an immunohistochemical study.

作者信息

Dagli Muharrem, Goksu Nebil, Eryilmaz Adil, Mocan Kuzey Gamze, Bayazit Yildirim, Gun Banu Dogan, Gocer Celil

机构信息

Department of Otolaryngology, Head and Neck Surgery, Ankara Numune Research and Training Hospital, Ankara, Turkey.

出版信息

Am J Otolaryngol. 2008 Jan-Feb;29(1):20-3. doi: 10.1016/j.amjoto.2006.12.003.

DOI:10.1016/j.amjoto.2006.12.003

PMID:18061827

Abstract

OBJECTIVE

The endolymphatic sac (ES) is part of the membranous labyrinth of the inner ear. Its central role in immunologic activity within the inner ear has been confirmed by numerous studies. The aim of this study was to investigate the expression of histamine receptors (H(1), H(2), H(3)) in the rabbit ES.

METHODS

A total of 10 healthy male New Zealand white rabbits weighing 2 to 3 kg were used in the experiments. For immunohistochemical studies, immunostaining was performed according to the avidin-biotin-peroxidase complex technique.

RESULTS

Serial sections of the ES of rabbits revealed the presence of H(1), H(2), and H(3) receptor immunoreactivity. Immunoreactive cells for all H(1), H(2), and H(3) were found in the epithelial and subepithelial layers of the duct and the proximal ES. In conclusion, this study showed the immunohistochemical localization of H(1), H(2), and H(3) receptors in the ES of rabbits. These receptors may be important in the homeostasis of the inner ear. In addition, they may be target receptors in the medical treatment of inner ear disorders such as endolymphatic hydrops.

摘要

目的

内淋巴囊（ES）是内耳膜迷路的一部分。众多研究已证实其在内耳免疫活动中的核心作用。本研究旨在探讨组胺受体（H(1)、H(2)、H(3)）在兔内淋巴囊中的表达。

方法

实验共使用10只体重2至3千克的健康雄性新西兰白兔。对于免疫组织化学研究，按照抗生物素蛋白-生物素-过氧化物酶复合物技术进行免疫染色。

结果

兔内淋巴囊的连续切片显示存在H(1)、H(2)和H(3)受体免疫反应性。在导管和近端内淋巴囊的上皮层和上皮下层中发现了所有H(1)、H(2)和H(3)的免疫反应性细胞。总之，本研究显示了H(1)、H(2)和H(3)受体在兔内淋巴囊中的免疫组织化学定位。这些受体可能在内耳稳态中起重要作用。此外，它们可能是内耳疾病如内淋巴积水医学治疗中的靶受体。