Nedachin A E, Lavrova D V, Dmitrieva R A, Doskina T V, Sanamian A G
Gig Sanit. 2007 Sep-Oct(5):68-70.
The paper describes an accelerated procedure for isolating enteric viruses and a scheme for evaluating the viral contamination of pure waters, which involves the concentration of the viruses from the great water volumes by means ofa MFM 0142 membrane module with tangential filtration through an electropositive membrane (MMK) with a pore size of 0.2 microm, followed eluent determination of RNA of viruses by RT-PCR in combination with coliphages or ICC by RT-PCR.
本文描述了一种分离肠道病毒的加速程序以及一种评估纯水病毒污染的方案,该方案包括通过MFM 0142膜组件从大量水中浓缩病毒,该膜组件通过孔径为0.2微米的正电膜(MMK)进行切向过滤,随后通过RT-PCR结合噬菌体或通过RT-PCR进行ICC对病毒RNA进行洗脱液测定。