Rezácová V, Baldrian P, Hrselová H, Larsen J, Gryndler M
Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
Folia Microbiol (Praha). 2007;52(4):415-21. doi: 10.1007/BF02932097.
Changes in microfungal communities, fungal activities and humic substances (HS) in agricultural soils kept under different fertilization regimes were observed and their causal relationships were investigated in a long-term field experiment. Fertilization did not change the abundance of HS-utilizing microfungi and, except for organic amendment alone, total culturable microfungi were also unaffected by this factor. Organic fertilization increased activities of manganese peroxidase (MnP) and proteinase, but decreased endo-1,4-beta-glucanase activity compared to the corresponding control without organic fertilization. In soils treated with mineral fertilizers, the activities of MnP, endo-1,4-beta-glucanase and proteinase were higher than in control without any mineral treatment. Both the aromaticity of fulvic acid and the molar mass of humic acid was lower in soil with organic fertilization, which may be a result of oxidative degradation mediated by higher MnP activity observed in treatments with organic fertilization.
在一项长期田间试验中，观察了不同施肥制度下农业土壤中微真菌群落、真菌活性和腐殖质（HS）的变化，并研究了它们之间的因果关系。施肥并未改变利用HS的微真菌的丰度，除了单独施用有机改良剂外，可培养的微真菌总数也不受该因素影响。与不施有机肥的相应对照相比，有机肥增加了锰过氧化物酶（MnP）和蛋白酶的活性，但降低了内切-1,4-β-葡聚糖酶的活性。在施用矿物肥料的土壤中，MnP、内切-1,4-β-葡聚糖酶和蛋白酶的活性高于未进行任何矿物处理的对照。施有机肥的土壤中富里酸的芳香性和腐殖酸的摩尔质量均较低，这可能是由于在有机肥处理中观察到较高的MnP活性介导的氧化降解所致。
