Zhou Ye-Qin, Yi Jian-Ping, Zhou Guo-Liang, Dong Ying
Department of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, China.
Wei Sheng Wu Xue Bao. 2007 Oct;47(5):817-22.
Tilletia indica Mitra, an important pest around the world which is the causative agent of Karnal Bunt of wheat, was very close to Tilletia walkeri phylogenetically and morphologically. 69.6 ng mitochondrial DNA (mtDNA) of Tilletia indica obtained from 6.4 mg total DNA by the method of CsCl/bisbenzimide density gradient ultracentrifugation can be used for electrophoresis, cloning, enzyme restriction analysis and PCR amplification. The ATP6 gene sequence was cloned from the fragment of mtDNA and sequenced for analysis of phylogenetic tree with the other related sequences in GenBank by the software PAUP to reveal the phylogenetic relationships within the Tilletia species.
印度腥黑粉菌（Tilletia indica Mitra）是一种在全球范围内重要的害虫，是小麦印度腥黑穗病的病原体，在系统发育和形态学上与沃氏腥黑粉菌（Tilletia walkeri）非常接近。通过氯化铯/双苯甲酰亚胺密度梯度超速离心法从6.4毫克总DNA中获得的69.6纳克印度腥黑粉菌线粒体DNA（mtDNA）可用于电泳、克隆、酶切分析和PCR扩增。从mtDNA片段中克隆出ATP6基因序列，并通过PAUP软件对其进行测序，以与GenBank中的其他相关序列分析构建系统发育树，从而揭示腥黑粉菌属物种内部的系统发育关系。
