Sun Changling, Xin Ding, Wang Yan
Department of Otolaryngology, the Fourth People' s Hospital, Wuxi, 214062, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 Sep;21(18):834-7.
To study the expressions of matrix metalloproteinase-2 (MMP-2) and E-cadherin in laryngeal carcinoma and their relationship with cervical lymph node metastasis.
The expression of MMP-2 and E-cadherin in 10 cases of normal laryngeal mucosa and 48 cases of supraglottic carcinoma were detected by immunohistochemistry.
The expression of MMP-2 in supraglottic carcinoma was significantly higher than that in normal laryngeal mucosa. The expression of MMP-2 in metastatic lymph nodes was significantly higher than that in nonmetastatic lymph nodes (P < 0.05). The expression of E-cadherin was significantly lower in supraglottic carcinoma than that in normal laryngeal mucosa. The expression of E-cadherin was significantly lower in metastatic lymph nodes than that in nonmetastatic lymph nodes (P < 0.05). There is a negative correlation between the expression of MMP-2 and E-cadherin (r = -0.41). Predicting the lymph node metastasis of laryngeal carcinoma with the index of MMP-2(+), E-cadherin(-) and MMP-2(+), E-cadherin(-). MMP-2(+) has the highest sensitivity, but the lowest specificity and positive predictive value. MMP-2(+), E-cadherin(-) has the highest specificity and positive predictive value, but the lowest sensitivity.
Expression of MMP-2 and E-cadherin can be used as a marker to predict lymph node metastasis of supraglottic carcinoma. Combined the detection of MMP-2 and E-cadherin can boost the accuracy of prediction of lymph node metastasis in supraglottic carcinoma and provide efficient assistance for resecting supraglottic carcinoma.
研究基质金属蛋白酶-2（MMP-2）和E-钙黏蛋白在喉癌中的表达及其与颈部淋巴结转移的关系。
采用免疫组织化学法检测10例正常喉黏膜组织和48例声门上型癌组织中MMP-2和E-钙黏蛋白的表达。
声门上型癌组织中MMP-2的表达明显高于正常喉黏膜组织。转移淋巴结中MMP-2的表达明显高于未转移淋巴结（P<0.05）。声门上型癌组织中E-钙黏蛋白的表达明显低于正常喉黏膜组织。转移淋巴结中E-钙黏蛋白的表达明显低于未转移淋巴结（P<0.05）。MMP-2与E-钙黏蛋白的表达呈负相关（r=-0.41）。以MMP-2（+）、E-钙黏蛋白（-）为指标预测喉癌淋巴结转移。MMP-2（+）的敏感性最高，但特异性和阳性预测值最低。MMP-2（+）、E-钙黏蛋白（-）的特异性和阳性预测值最高，但敏感性最低。
MMP-2和E-钙黏蛋白的表达可作为预测声门上型癌淋巴结转移的指标。联合检测MMP-2和E-钙黏蛋白可提高声门上型癌淋巴结转移预测的准确性，为声门上型癌的手术切除提供有效帮助。
