Hine P M, Engelsma M Y, Wakefield St J
Investigation and Diagnostic Centre, Biosecurity New Zealand, Ministry of Agriculture and Forestry, PO Box 40-742, Upper Hutt 6007, New Zealand.
Dis Aquat Organ. 2007 Oct 15;77(3):225-33. doi: 10.3354/dao01822.
An ultrastructural study was carried out on the tissues of an oyster (Ostrea edulis), heavily infected with Haplosporidium armoricanum, that had been fixed in Carson's fixative. The well-fixed tissues revealed details of sporulation and of the spores, which had not been previously reported from H. armoricanum. These include the initial presence of sparse haplosporosomes after thickening of the plasma membrane in early sporonts, division of sporont nuclei by multiple fission, cup-like indentations in the nuclear surface associated with putative nuclear material in both the sporonts and spores, and cytoplasmic multi-vesicular bodies in the cytoplasm of sporonts and spores. The spore wall and operculum were formed from a light matrix that occurred in short cisternae of smooth endoplasmic reticulum in the episporoplasm, and parallel bundles of microfibrils were present in some spores. Spores were rarely bi-nucleate with the nuclei occurring as a diplokaryon, with putative nuclear material at the junction of the 2 nuclei. Nuclear membrane-bound Golgi (NM-BG) cisternae were common in spores, and they appeared to synthesise a light granular material into lysosome-like granules. Dense bodies similar to those reported from H. lusitanicum, H. pickfordi and H. monforti occurred in, or outside, the peripheral endosporoplasm, which was closely apposed to the spore wall. Spore haplosporosomes were frequently axehead-shaped, more like those of H. costale than those previously reported from H. armoricanum, and in some haplosporosomes there was a small round lucent patch with a dark point near the centre of the lucent patch. Overall, H. armoricanum appears to be closely related to H. costale and Bonamia spp. Although the endosporoplasm of H. armoricanum has NM-BG and it resembles the uni-nucleate stage, it appears to be unlikely that they are the same, as the axehead-shaped haplosporosomes of the spore differ considerably from the spherical haplosporosomes of vegetative stages.
对一只严重感染了阿摩利单孢子虫（Haplosporidium armoricanum）的食用牡蛎（Ostrea edulis）组织进行了超微结构研究，该牡蛎已用卡森固定液固定。固定良好的组织揭示了孢子形成和孢子的细节，这些细节此前尚未在阿摩利单孢子虫中报道过。这些细节包括早期孢子母细胞中质膜增厚后稀疏的单孢子体的最初出现、孢子母细胞核通过多次分裂进行分裂、孢子母细胞和孢子中核表面与假定核物质相关的杯状凹陷，以及孢子母细胞和孢子细胞质中的细胞质多囊体。孢子壁和盖由一种浅色基质形成，该基质出现在孢子周质中光滑内质网的短池中，并且在一些孢子中存在平行的微纤丝束。孢子很少双核，细胞核以双核体形式出现，在两个细胞核的交界处有假定的核物质。核膜结合的高尔基体（NM - BG）池在孢子中很常见，并且它们似乎将一种浅色颗粒物质合成到溶酶体样颗粒中。类似于在葡萄牙单孢子虫（H. lusitanicum）、皮氏单孢子虫（H. pickfordi）和蒙氏单孢子虫（H. monforti）中报道的致密体出现在外周内孢子质中或其外部，外周内孢子质与孢子壁紧密相邻。孢子单孢子体通常呈斧形，比之前在阿摩利单孢子虫中报道的更像科氏单孢子虫（H. costale）的，并且在一些单孢子体中，在透明斑的中心附近有一个带有暗点的小圆形透明斑。总体而言，阿摩利单孢子虫似乎与科氏单孢子虫和博纳米亚属（Bonamia spp.）密切相关。尽管阿摩利单孢子虫的内孢子质有NM - BG并且它类似于单核阶段，但它们似乎不太可能相同，因为孢子的斧形单孢子体与营养阶段的球形单孢子体有很大差异。
