Egwari L O, Rotimi V O
Dept of Medical Microbiology and Parasitology, College of Medicine University of Lagos, Idi-Araba, Nigeria.
Cent Afr J Med. 1991 Dec;37(12):415-9.
The viability of four Bacteroides species in Amies medium at different temperatures was evaluated by a series of quantitative and qualitative experiments using B. bivius, B. disiens, B. fragilis and B. melaninogenicus. In the quantitative assessment, the Bacteroides spp. were recovered from Amies transport medium stored at -20 degrees C up to the six weeks of storage. At -10 degrees C no Bacteroides spp. was recovered after two weeks. At both storage temperatures, B. fragilis showed the highest survival rate. In the qualitative assessment, the test strains were recovered after four months of storage at -20 degrees C in Amies medium with noticeable decline in number of surviving bacteria towards the end of that period. The effect of thawing and refreezing was also assessed and was found to affect survival rate considerably. Our results provide an alternative, convenient and cheaper method of preserving anaerobes.
通过一系列定量和定性实验，使用双栖拟杆菌、双酶拟杆菌、脆弱拟杆菌和产黑素拟杆菌，评估了四种拟杆菌属细菌在不同温度下于阿氏培养基中的生存能力。在定量评估中，拟杆菌属细菌可从储存于-20℃长达六周的阿氏运输培养基中复苏。在-10℃时，两周后未复苏出拟杆菌属细菌。在这两个储存温度下，脆弱拟杆菌的存活率最高。在定性评估中，测试菌株在阿氏培养基中于-20℃储存四个月后可复苏，在此期间结束时存活细菌数量显著下降。还评估了解冻和重新冷冻的影响，发现其对存活率有相当大的影响。我们的结果提供了一种保存厌氧菌的替代、方便且更便宜的方法。
