Eto S, Noda H, Minemoto M, Noda A, Mizukami Y
Department of Hospital Pharmacy, School of Medicine, University of Occupational and Environmental Health, Japan.
Chem Pharm Bull (Tokyo). 1991 Oct;39(10):2742-4. doi: 10.1248/cpb.39.2742.
Both S-(-)- and R-(+)-enantiomers of 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), a main oxidative metabolite of the achiral antiepileptic drug phenytoin, could be determined simply, sensitively and accurately using reversed phase high-performance liquid chromatography by using a methanol-monopotassium phosphate eluent containing beta-cyclodextrin. Using this assay procedure, it was determined that an S-(-)-enantiomer was formed predominantly by the oxidation of phenytoin in isolated rat hepatocytes.
