Minami C, Deng Y, Urano F, Kaiya T, Kohda K, Kawai M, Naoi M
Department of Applied Chemistry, Nagoya Institute of Technology, Japan.
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):245-8. doi: 10.1016/s0378-4347(97)00374-5.
A new preparation method was devised using beta-cyclodextrin as a mobile-phase additive in a reversed-phase high-performance liquid chromatography system. Preparative separation of the biologically active (R)-enantiomer was achieved from racemic N-methylsalsolinol. Beta-cyclodextrin was removed completely in good yield by acid extraction and solid-phase extraction. By a slight modification, this method will be applicable to the isolation of various types of biologically important enantiomers.
设计了一种新的制备方法，即在反相高效液相色谱系统中使用β-环糊精作为流动相添加剂。从外消旋N-甲基去甲猪毛菜碱中实现了具有生物活性的（R）-对映体的制备分离。通过酸萃取和固相萃取以高收率完全除去了β-环糊精。稍加修改，该方法将适用于分离各种类型的具有生物学重要意义的对映体。
