Triantafyllou K, Kitsanta P, Karamanolis D G, Kittas C, Ladas S D
Hepatogastroenterology Unit, 2nd Department of Internal Medicine, Attikon University General Hospital, Medical School, Athens University, 1 Rimini Street, 12462 Haidari, Athens, Greece.
Dig Liver Dis. 2008 Jan;40(1):39-45. doi: 10.1016/j.dld.2007.09.010. Epub 2007 Dec 11.
To investigate epithelial cell turnover alterations, and p53, bcl-2 protein expression during development of early and advanced gastric cancer in a Western population.
We investigated cell apoptosis and proliferation rates, p53 and bcl-2 protein expression in 17 early and 34 advanced gastric carcinomas and in the adjacent non-dysplastic mucosa. Cell proliferation, p53 and bcl-2 expression were detected immunohistochemically using MIB-1, anti-p53 and anti-bcl-2 monoclonal antibodies. Apoptosis was measured by TUNEL. The rate of the positive stained cells (labelling index) was count using image analysis technique.
No difference was observed of either apoptotic (10 vs. 11) or proliferation (35 vs. 25) index between early and advanced cancers. However, the apoptotic index was significantly higher in intestinal type advanced tumors. While both apoptotic and proliferation indices were significantly higher in tumors than in the adjacent mucosa, no difference was observed of either apoptotic (2 vs. 2) or proliferation (8 vs. 13) index between the tissues adjacent to early and advanced tumors. p53 protein expression was significantly higher in advanced cancers (7 vs. 5, p=0.001) and in the non-dysplastic tissue adjacent to advanced tumors (3.5 vs. 2, p=0.001). bcl-2 labelling index was significantly higher in the mucosa adjacent to advanced carcinomas (15 vs. 5, p=0.016) but this difference did not reach significance in the tumors (20 vs. 15, p=0.37).
Our data indicate similar cell turnover during tumorigenesis of early and advanced cancer. p53 and bcl-2 protein accumulation is more intense in gastric mucosa adjacent to advanced tumors and p53 immunoreactivity peaks in advanced carcinomas.
研究西方人群早期和进展期胃癌发生过程中上皮细胞更新变化以及p53、bcl-2蛋白表达情况。
我们调查了17例早期和34例进展期胃癌及其相邻的非发育异常黏膜中的细胞凋亡和增殖率、p53和bcl-2蛋白表达。使用MIB-1、抗p53和抗bcl-2单克隆抗体通过免疫组织化学法检测细胞增殖、p53和bcl-2表达。通过TUNEL法检测凋亡情况。使用图像分析技术计算阳性染色细胞率（标记指数）。
早期和进展期癌症之间的凋亡指数（10对11）或增殖指数（35对25）均未观察到差异。然而，肠型进展期肿瘤的凋亡指数显著更高。虽然肿瘤中的凋亡和增殖指数均显著高于相邻黏膜，但早期和进展期肿瘤相邻组织之间的凋亡指数（2对2）或增殖指数（8对13）均未观察到差异。进展期癌症（7对5，p=0.001）以及进展期肿瘤相邻的非发育异常组织（3.5对2，p=0.001）中p53蛋白表达显著更高。进展期癌相邻黏膜中的bcl-2标记指数显著更高（15对5，p=0.016），但在肿瘤中这种差异未达到显著水平（20对15，p=0.37）。
我们的数据表明早期和进展期癌症发生过程中细胞更新情况相似。p53和bcl-2蛋白在进展期肿瘤相邻的胃黏膜中积累更强烈，且p53免疫反应性在进展期癌中达到峰值。
