[胃癌前病变中细胞凋亡与增殖的相关性]

[Correlation between apoptosis and proliferation in gastric pre-carcinoam].

作者信息

Xu A, Li S, Liu J

机构信息

Research Laboratory of Digestive Disease, Huizhou Central People's Hospital, Guangdong 516001.

出版信息

Zhonghua Yi Xue Za Zhi. 1999 Mar;79(3):185-6.

PMID:11601036

Abstract

OBJECTIVE

To clarify the correlation between apoptosis/proliferation and the expressions of oncogene bcl-2 and P53 in the changes from superficial gastritis, intestinal metaplasia, dysplasia, gastric cancer in early stage to advanced gastric cancer.

METHODS

TUNEL method was used to detected apoptosis. Immunohistochemical analysis was used to detect the expressions of proliferating cellular nuclear antigen(PCNA), bcl-2 protein and P53 protein. Results In the patients of superficial gastritis, atrophic gastritis, intestinal metaplasia, dysplasia, early gastric cancer and advanced gastric cancer and advanced gastric cancer, apoptotic indexes were: (6.4 +/- 1.9)%, (14.1 +/- 4.1)%, (23.3 +/- 6.1)%, (17.4 +/- 2.6)%, (11.3 +/- 3.7)%, (6.3 +/- 2.0)% respectively. PCNA indexes were: (11.3 +/- 2.2)%, (18.9 +/- 6.5)%, (20.3 +/- 7.3)%, (40.0 +/- 10.6)%, (53.1 +/- 10.9)% and (72.4 +/- 18.4)% respectively; the expression rates of bcl-2 were 10.0%, 23.3%, 40.0%, 56.7%, 85.7% and 46.7%. The expression rates of P53 were: 0%, 0%, 0%, 4.3%, 14.3% and 53.3%. The difference in the majority of observed indexes were significant (P < 0.05-0.01).

CONCLUSION

During the changes of gastric cancer, the apoptotic and proliferating indexes incerased simultaneously in superficial gastritis, atrophic gastritis and intestinal metaplasia. However, PCNA indexes further increased but apoptotic indexes decreased in dysplasia, early gastric cancer and advanced gastric cancer and advanced gastric cancer. The former may be the phenomenon in early gastric cancer and advanced gastric cancer and advanced gastic cancer. The former may be the phenomenon in ear early gastric cancer the latter may be the phenomenon in advanced gastric cancer.

摘要

目的

阐明从浅表性胃炎、肠化生、发育异常、早期胃癌到进展期胃癌的病变过程中细胞凋亡/增殖与癌基因bcl-2和P53表达之间的相关性。

方法

采用TUNEL法检测细胞凋亡。采用免疫组织化学分析法检测增殖细胞核抗原（PCNA）、bcl-2蛋白和P53蛋白的表达。结果浅表性胃炎、萎缩性胃炎、肠化生、发育异常、早期胃癌和进展期胃癌患者的凋亡指数分别为：(6.4±1.9)%、(14.1±4.1)%、(23.3±6.1)%、(17.4±2.6)%、(11.3±3.7)%、(6.3±2.0)%。PCNA指数分别为：(11.3±2.2)%、(18.9±6.5)%、(20.3±7.3)%、(40.0±10.6)%、(53.1±10.9)%和(72.4±18.4)%；bcl-2的表达率分别为10.0%、23.3%、40.0%、56.7%、85.7%和46.7%。P53的表达率分别为：0%、0%、0%、4.3%、14.3%和53.3%。大多数观察指标的差异具有显著性（P<0.05-0.01）。