Xie Song, Sun Lina, Liu Fengsong, Dong Bo
College of Life Sciences, Hebei University, Baoding 071002, China.
Mol Biol Rep. 2009 Feb;36(2):389-97. doi: 10.1007/s11033-007-9192-1. Epub 2007 Dec 7.
A full-length cDNA encoding vitellogenin (Vg) was cloned from Chinese shrimp, Fenneropenaeus chinensis using RACE method. The full-length cDNA consist of 7,942 nucleotides including a 7,761 bp open reading frame, which encodes 2,587 amino acid residues. The deduced amino acid sequence showed high (from 94% to 37%) identity with other known crustacean Vgs. In addition, a consensus cleavage site (R-X-K/R-R) recognized by an endopeptidase and a member of subtilisin family of serine protease were identified in the deduced Vg precursor. RT-PCR analysis shown that Vg mRNA can be detected in both ovary and hepatopancreas of vitellogenic females but not in other experimental tissues including muscle, heart, lymph organ, gill, haemocytes and intestine. These results suggest that the Vg gene may be expressed exclusively in mature females, and both ovary and hepatopancreas are the possible tissues for Vg synthesis in F. chinensis. In addition, Vg gene is detected in genomic DNA of both females and males.
采用RACE方法从中国对虾（凡纳滨对虾）中克隆了一个编码卵黄蛋白原（Vg）的全长cDNA。该全长cDNA由7942个核苷酸组成，其中包括一个7761 bp的开放阅读框，编码2587个氨基酸残基。推导的氨基酸序列与其他已知甲壳类动物的Vg具有较高的同源性（94%至37%）。此外，在推导的Vg前体中鉴定出一个被内肽酶识别的共有切割位点（R-X-K/R-R）以及丝氨酸蛋白酶枯草杆菌蛋白酶家族的一个成员。RT-PCR分析表明，在卵黄发生期雌虾的卵巢和肝胰腺中均可检测到Vg mRNA，但在包括肌肉、心脏、淋巴器官、鳃、血细胞和肠道在内的其他实验组织中未检测到。这些结果表明，Vg基因可能仅在成熟雌虾中表达，卵巢和肝胰腺都是中国对虾中Vg合成的可能组织。此外，在雌性和雄性的基因组DNA中均检测到Vg基因。
