Kruanetr Senee, Tengjaroenkul Urai, Liawruangrath Boonsom, Liawruangrath Saisunee
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Oct;70(5):1134-40. doi: 10.1016/j.saa.2007.10.034. Epub 2007 Nov 6.
A cleaner and simple spectrophotometric method using microflow analysis (muFA) was performed. It consisted of a T-junction with microcoil on a polymethyl methacrylate (PMMA) chip which was fabricated by laser ablation and a molded polydimethylsiloxane (PDMS) as top plate. The fabricated PMMA chip was integrated with light emitting diode (LED) as light source and spectrometer as detector. The proposed device was applied to determining copper in water samples using nitroso-R salt as chromogenic reagent at 495 nm. It was found that the proposed muFA system was with less reagents and samples consumption with tiny waste generation. The relative standard deviation (R.S.D.) was less than 2% (n=11) with the percentage recovery of 98.0+/-1.7% (n=7). The linear range for determination of copper in water samples was over the range of 0.05-3.0 microg mL(-1) with a correlation coefficient (r2) of 0.999. The limit of detection (3sigma) was 47 ng mL(-1) with a sample throughput of 30 h(-1).
采用微流分析（μFA）进行了一种更清洁、简单的分光光度法。它由一个在聚甲基丙烯酸甲酯（PMMA）芯片上带有微线圈的T型接头组成，该芯片通过激光烧蚀制造，顶板为模制聚二甲基硅氧烷（PDMS）。所制造的PMMA芯片与作为光源的发光二极管（LED）和作为检测器的光谱仪集成在一起。该装置用于以亚硝基-R盐为显色剂，在495nm波长下测定水样中的铜。结果表明，所提出的μFA系统试剂和样品消耗少，产生的废物极少。相对标准偏差（R.S.D.）小于2%（n = 11），回收率为98.0±1.7%（n = 7）。水样中铜的测定线性范围为0.05 - 3.0 μg mL-1，相关系数（r2）为0.999。检测限（3σ）为47 ng mL-1，样品通量为30 h-1。
