Li Feng, Wu Yi-hui, Zhao Hua-bing, Ju Hui
Changchun Institute of Optics, Fine Mechanics and Physics, State Key Lab of Applied Optics, Changchun 130033, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Apr;25(4):633-6.
For the need of biochemical chip, which consumes fewer specimens and is easy to integrate with micro-fluid chip, two kinds of spectrophotometric analysis methods are described in the present paper. Both the direct detection method and evanescent wave detection method are used in the experiments with visible light (460-800 nm). The experimental results proved that the direct detection is simple and evident; on the other hand the evanescent wave detection method consumes much less reagent and is easy to integrate with microchips.
针对生化芯片需要消耗较少样本且易于与微流控芯片集成的需求,本文介绍了两种分光光度分析方法。直接检测法和倏逝波检测法都用于可见光(460 - 800 nm)的实验中。实验结果表明,直接检测法简单明了;另一方面,倏逝波检测法消耗的试剂少得多,且易于与微芯片集成。