Gartner A, Carles C, Montagne P, Cuillière M L, Duheille J
Laboratoire de Nutrition Tropicale de l'ORSTOM, Montpellier, France.
J Immunoassay. 1991;12(4):521-42. doi: 10.1080/01971529108053278.
This article describes a microparticle enhanced nephelometric immunoassay (Nephelia) applied to the quantification of the thymic peptide hormone thymulin. Nephelia uses antibody recognition by the antithymulin antiserum in a competitive reaction between free thymulin and thymulin bound to the microspheres. The binding between microsphere and thymulin is achieved with the aid of a protein carrier. The sensitivity of the competitive reaction varied with the protein carrier and the antiserum sample. The most efficient reaction was obtained with the thymulin-metallothionein-microsphere conjugates; as little as 5 pg/ml of thymulin could be detected. In adult human serum or in its ultra-filtrate, spiked synthetic thymulin was totally recovered. Measurement of thymulin in serum or ultra-filtrate samples demonstrated the presence of interference from molecules in the serum. Nephelia led to the same conclusions as those reported with other methods and performs as well, and is more simple to use than radio- or enzymo-immunoassays for thymulin measurement.
本文描述了一种用于定量胸腺肽激素胸腺生成素的微粒增强散射比浊免疫分析方法（Nephelia）。Nephelia利用抗胸腺生成素抗血清在游离胸腺生成素与结合在微球上的胸腺生成素之间的竞争性反应中进行抗体识别。微球与胸腺生成素之间的结合借助蛋白质载体实现。竞争性反应的灵敏度随蛋白质载体和抗血清样品而变化。使用胸腺生成素-金属硫蛋白-微球缀合物可获得最有效的反应；可检测到低至5 pg/ml的胸腺生成素。在成人血清或其超滤液中，添加的合成胸腺生成素可完全回收。对血清或超滤液样品中胸腺生成素的测量表明血清中的分子存在干扰。Nephelia得出的结论与其他方法报告的结论相同，性能良好，并且比用于测量胸腺生成素的放射免疫分析或酶免疫分析更易于使用。
