Marchand J, Varcin P, Riochet D, Montagne P, Cuilliere M L, Duheille J, Pau B
Sanoci Research, Montpellier.
Biopolymers. 1992 Aug;32(8):971-80. doi: 10.1002/bip.360320808.
To optimize antigen-antibody reactions, we have synthesized chemically well-defined hydrophilic microspheres. Proteins or haptens were covalently linked to these carriers. When the microsphere conjugates were agglutinated by the corresponding antiserum, the size of the complex artificially increased during the immunological reaction. After optimizing various parameters such as the hydrophilic character, repulsion charges, and amount of antigen coupled to the microspheres, we developed a rapid and sensitive immunoassay based on laser light scattering by the complexes.
为优化抗原 - 抗体反应，我们合成了化学性质明确的亲水性微球。蛋白质或半抗原被共价连接到这些载体上。当微球偶联物被相应抗血清凝集时，在免疫反应过程中复合物的大小会人为增加。在优化了诸如亲水性、排斥电荷以及偶联到微球上的抗原量等各种参数后，我们开发了一种基于复合物激光散射的快速灵敏免疫测定法。
