利用介观荧光断层扫描对黑腹果蝇蛹进行体内成像。
In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.
作者信息
Vinegoni Claudio, Pitsouli Chrysoula, Razansky Daniel, Perrimon Norbert, Ntziachristos Vasilis
机构信息
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA.
出版信息
Nat Methods. 2008 Jan;5(1):45-7. doi: 10.1038/nmeth1149. Epub 2007 Dec 9.
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
我们报告了一种荧光断层扫描技术，该技术突破了组织切片荧光显微镜的穿透极限。该方法采用多投影照明和不透明组织中的光子传输描述。我们展示了活体黑腹果蝇蛹体内绿色荧光蛋白（GFP）表达的唾液腺和翅成虫盘形态发生的全身三维可视化，并观察了其随时间的变化。
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