聚酰胺-胺型（PAMAM）G2和G6树枝状大分子对牛血清白蛋白（游离脂肪酸及负载不同脂肪酸的牛血清白蛋白）的影响。
Impact of PAMAM G2 and G6 dendrimers on bovine serum albumin (fatty acids free and loaded with different fatty acids).
作者信息
Shcharbin Dzmitry, Ottaviani M Francesca, Cangiotti Michela, Przybyszewska Magdalena, Zaborski Marian, Bryszewska Maria
机构信息
Department of General Biophysics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
出版信息
Colloids Surf B Biointerfaces. 2008 May 1;63(1):27-33. doi: 10.1016/j.colsurfb.2007.10.020. Epub 2007 Nov 4.
Dendrimers are a new class of nanotechnological polymers suitable for drug targeting, microarray systems or detoxication. The present study is devoted to a detailed analysis of binding between PAMAM dendrimers and bovine serum albumin (fatty acid free or loaded with oleic, linoleic, oleic+linoleic or oleic+linoleic+arachodonic acids) by measuring zeta-potential, fluorescence quenching, fluorescence anisotropy and electron paramagnetic resonance. Addition of PAMAM G2 and G6 dendrimers to protein solutions resulted in attachment to the protein molecule. The PAMAM dendrimers also competed with BSA for fatty acids if two or three fatty acids were loaded per protein. This can lead to the extraction of fatty acids from BSA to the PAMAM dendrimer.
树枝状聚合物是一类新型的纳米技术聚合物，适用于药物靶向、微阵列系统或解毒。本研究致力于通过测量zeta电位、荧光猝灭、荧光各向异性和电子顺磁共振，详细分析聚酰胺-胺型（PAMAM）树枝状聚合物与牛血清白蛋白（无脂肪酸或负载油酸、亚油酸、油酸+亚油酸或油酸+亚油酸+花生四烯酸）之间的结合。向蛋白质溶液中添加PAMAM G2和G6树枝状聚合物会导致其附着在蛋白质分子上。如果每个蛋白质负载两个或三个脂肪酸，PAMAM树枝状聚合物还会与牛血清白蛋白竞争脂肪酸。这可能导致脂肪酸从牛血清白蛋白中被提取到PAMAM树枝状聚合物中。
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