Liu Zhong-Quan, Li Chuan-You, Chen Xiao-You, Chen Xi, Ma Yu
Department of Bacteriology and Immunology, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Jul;28(7):697-9.
To establish a rapid method for testing drug sussceptibility on Mycobacterium tuberculosis.
Taking absolute Concentration method for drug susceptibility testing of M. tuberculosis as the "gold standard", we examined the drug-resistant of M. tuberculosis strain with nitrate reducrase assay (NRA) and the drug-resistant of M. tuberculosis germ in sputum with NRA.
NRA and absolute concentration method was basically comparable with NRA susceptibility as 96.5% and the specificity was 100%, When comparing with traditional absolute concentration method, NRA could shorten the time about 3 weeks. Using NRA to test the drug-resistant of M. tuberculosis germ in sputum, its susceptibility was more than 66.7% and specificity was 100%, within 10-20 days.
NRA could be used as a rapid drug susceptibility testing on M. tuberculosis.
