Jiang Wen-shan, Yin Zheng-qin
The Southwest Ophthalmology Hospital of the Third Military Medical University, Chongqing 400038, China.
Zhonghua Yan Ke Za Zhi. 2007 Sep;43(9):823-8.
To screening genes associated with termination of the critical period of neuroplasticity in visual cortex and investigate the molecular mechanism.
To construct a cDNA library using visual cortex of dark rearing rats and normal light rearing 1 week after dark rearing 60 days rats with suppression subtractive hybridization technique. PCR, reverse Northern hybridization, sequencing and homology search were used to analysis the differential expression genes fragments.
The cDNA library of termination the critical period was set up successfully. After screening, sequencing and homology search 14 sequences were obtained. Among of the genes 13 were known genes and one was novel gene fragment. They were up-regulated genes in visual cortex when the critical period termination.
Through construction the cDNA library, we screened a crop of candidate genes related to termination the critical period. The results provided important foundation to further illuminate the molecular mechanism of termination the critical period of neuroplasticity in visual cortex.
筛选与视觉皮层神经可塑性关键期终止相关的基因，并探讨其分子机制。
采用抑制性消减杂交技术，构建暗饲养60天后暗饲养1周的大鼠视觉皮层与正常光照饲养大鼠视觉皮层的cDNA文库。利用PCR、反向Northern杂交、测序及同源性搜索分析差异表达基因片段。
成功构建了关键期终止的cDNA文库。经过筛选、测序及同源性搜索，获得14个序列。其中13个为已知基因，1个为新基因片段。它们是关键期终止时视觉皮层中的上调基因。
通过构建cDNA文库，筛选出一批与关键期终止相关的候选基因。这些结果为进一步阐明视觉皮层神经可塑性关键期终止的分子机制提供了重要基础。
