Patel Sanjay V, Erie Jay C, McLaren Jay W, Bourne William M
Department of Ophthalmology, Mayo Clinic College of Medicine, 200 First St SW, Rochester, MN 55905, USA.
Arch Ophthalmol. 2007 Dec;125(12):1693-8. doi: 10.1001/archopht.125.12.1693.
To determine central keratocyte and subbasal nerve densities in clear and failed grafts after penetrating keratoplasty.
Clear grafts and grafts with late endothelial failure (LEF) were examined using confocal microscopy 1 to 31 years after penetrating keratoplasty. Keratocyte density, number of keratocytes in a full-thickness column of stroma, and subbasal nerve density were determined from images. Comparisons were made with normal corneas.
The mean +/- SD keratocyte density in clear grafts (22 101 +/- 3799 cells/mm(3)) was lower than that in normal corneas (26 610 +/- 3683 cells/mm(3); P < .001) but did not differ from that in grafts with LEF (21 268 +/- 3298 cells/mm(3); P = .47). The mean +/- SD number of keratocytes in clear grafts (10 325 +/- 1708 cells) was lower than that in normal corneas (11 466 +/- 1503 cells; P < .001) but did not differ from that in grafts with LEF (10 778 +/- 1760 cells; P = .39). Median subbasal nerve density in clear grafts (150 microm/mm(2)) was lower than that in normal corneas (7025 microm/mm(2); P < .001), and nerve recovery correlated with time after surgery (r = 0.36; P < .001).
Keratocyte density and number are decreased in penetrating grafts compared with normal corneas. Subbasal nerve density does not recover to normal through 3 decades.
确定穿透性角膜移植术后透明植片和失败植片中中央角膜细胞及基底膜下神经密度。
对穿透性角膜移植术后1至31年的透明植片和发生晚期内皮功能衰竭（LEF）的植片进行共聚焦显微镜检查。从图像中确定角膜细胞密度、基质全层柱中的角膜细胞数量以及基底膜下神经密度。与正常角膜进行比较。
透明植片中角膜细胞的平均±标准差密度（22 101±3799个细胞/mm³）低于正常角膜（26 610±3683个细胞/mm³；P<.001），但与发生LEF的植片（21 268±3298个细胞/mm³；P = .47）无差异。透明植片中角膜细胞的平均±标准差数量（10 325±1708个细胞）低于正常角膜（11 466±1503个细胞；P<.001），但与发生LEF的植片（10 778±1760个细胞；P = .39）无差异。透明植片中基底膜下神经密度中位数（150μm/mm²）低于正常角膜（7025μm/mm²；P<.001），且神经恢复与术后时间相关（r = 0.36；P<.001）。
与正常角膜相比，穿透性移植片中角膜细胞密度和数量降低。基底膜下神经密度在30年内未恢复至正常水平。
