Kanazawa Masanori, Sato Shinobu, Ohtsuka Keiichi, Takenaka Shigeori
Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu, Fukuoka 840-8550, Japan.
Anal Sci. 2007 Dec;23(12):1415-9. doi: 10.2116/analsci.23.1415.
Messenger RNA (mRNA) poly(A)+RNA (from mouse kidney) was immobilized on a N-hydroxysuccinimide(NHS)-activated carboxylic acid modified electrode prepared by the treatment of a gold electrode with 3,3'-dithiodipropionic acid, followed by NHS and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). An electrochemical measurement using this mRNA electrode was carried out in an electrolyte containing ferrocenylnaphthalene diimide (1), and showed an electrochemical signal based on 1 concentrated on immobilized mRNA. After treating this electrode with water containing varied amounts of ribonuclease A (RNase A), the current peak based on 1 decreased with increasing in the amount of RNase A with a linear correlation in the range of 0.2-10 pg of RNase A.
信使核糖核酸（mRNA）聚腺苷酸加尾RNA（来自小鼠肾脏）被固定在通过用3,3'-二硫代二丙酸处理金电极，随后用N-羟基琥珀酰亚胺（NHS）和1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）制备的N-羟基琥珀酰亚胺（NHS）活化的羧酸修饰电极上。使用该mRNA电极在含有二茂铁基萘二酰亚胺（1）的电解质中进行电化学测量，结果显示基于1浓缩在固定化mRNA上的电化学信号。在用含有不同量核糖核酸酶A（RNase A）的水处理该电极后，基于1的电流峰随着RNase A量的增加而降低，在0.2 - 10 pg RNase A范围内呈线性相关。
