Quantitation of microbial metabolism.

Quantitation is a characteristic property of natural sciences and technologies and is the background for all kinetic and dynamic studies of microbial life. This presentation concentrates therefore on materials and methods as tools necessary to accomplish a sound, quantitative and mechanistic understanding of metabolism. Mathematical models are the software, bioreactors, actuators and analytical equipment are the hardware used. Experiments must be designed and performed in accordance with the relaxation times of the biosystem investigated; some of the respective consequences are discussed and commented in detail. Special emphasis is given to the required density, accuracy and reproducibility of data as well as their validation.