Tyther Raymond, Ahmeda Ahmad, Johns Edward, Sheehan David
Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland.
Proteomics. 2007 Dec;7(24):4555-64. doi: 10.1002/pmic.200700503.
Nitrosative and oxidative stress are implicated in the development of hypertension. Events in the renal medulla may play a key role in the development and progression of hypertension. This may arise through disruption of nitric oxide signalling in the medulla and be accompanied by enhanced nitrosative and oxidative stress as indicated by the presence of proteins containing 3-nitrotyrosine. Here we demonstrate enhanced protein nitration in the medulla of spontaneously hypertensive rats. We have identified several nitrated proteins with both varied subcellular location and functional roles. These proteins are involved in nitric oxide signalling, antioxidant defense and energy metabolism. Moreover, increased nitration was observed in conjunction with enhanced oxidative damage as evidenced by the presence of protein carbonyl oxidative stress biomarkers. Our results suggest that kidney medulla is subject to enhanced nitrosative and oxidative stress, and that resulting protein modifications may contribute to the progression of hypertension.
亚硝化应激和氧化应激与高血压的发生发展有关。肾髓质中的事件可能在高血压的发生和进展中起关键作用。这可能是由于髓质中一氧化氮信号传导的破坏引起的，并伴随着亚硝化和氧化应激的增强，含3-硝基酪氨酸的蛋白质的存在表明了这一点。在这里，我们证明了自发性高血压大鼠髓质中蛋白质硝化作用增强。我们已经鉴定出几种具有不同亚细胞定位和功能作用的硝化蛋白质。这些蛋白质参与一氧化氮信号传导、抗氧化防御和能量代谢。此外，正如蛋白质羰基氧化应激生物标志物的存在所证明的，硝化作用增加与氧化损伤增强同时出现。我们的结果表明，肾髓质受到增强的亚硝化和氧化应激，由此产生的蛋白质修饰可能有助于高血压的进展。
