Tanaka K, Atwell R B
Department of Companion Animal Medicine and Surgery, University of Queensland, St Lucia.
Aust Vet J. 1991 Dec;68(12):396-9. doi: 10.1111/j.1751-0813.1991.tb03108.x.
Plasma samples from dogs with infections of Dirofilaria immitis were assessed using ELISA and Western blotting techniques. These results were then assessed in relation to age and sex of the host, and to the numbers of microfilariae and adult filariae. Dogs with microfilariae tended to have lower levels of infection. In infected dogs, mean ELISA titres increased directly with the degree of infection. Dogs that were either young or mildly infected, showed a preferential antibody reactivity to antigens in the high molecular weight regions of the immunoblots. With increasing age and/or the extent of infection, an antibody response to antigens in the low molecular weight regions was apparent.
使用酶联免疫吸附测定（ELISA）和蛋白质印迹技术评估感染犬恶丝虫的犬的血浆样本。然后根据宿主的年龄和性别以及微丝蚴和成虫丝虫的数量对这些结果进行评估。有微丝蚴的犬感染水平往往较低。在受感染的犬中，ELISA平均滴度随感染程度直接升高。年轻或轻度感染的犬对免疫印迹高分子量区域的抗原表现出优先的抗体反应性。随着年龄增长和/或感染程度增加，对低分子量区域抗原的抗体反应明显。
